- 【专题讨论】流式细胞术专区 2025/01/03
最近做流式,检测正常人及患者PBMNC各亚群Tim-3的表达,用三色标记,其中(1)管为FITC-CD4/PE-Tim-3/PE-Cy5-CD3;(2)管为FITC-CD8/PE-Tim-3/PE-Cy5-CD3;(3)管为FITC-CD14/PE-Tim-3/PE-Cy5-CD3;(4)管为FITC-CD16+CD56/PE-Tim-3/PE-Cy5-CD3;(5)管为FITC-CD19/PE-Tim-3/PE-Cy5-CD3。操作步骤如下:(1)Falcon管分别标记,加入荧光标记的单克隆...